Folders becoming read-only has been a problem for many users who desperately want to get rid of it and you're out trying to do it. This link discusses the problem: Link Removed
One of the suggested solutions is to use the DOS attrib command to turn read-only off (in your case, on). It isn't clear to me why they would do that from a command line rather than just using Properties in Windows Explorer. However, setting a folder to read-only just makes the files within it read-only, not the folder, itself.
It isn't clear from your post where files are being added and where you want to use the folder. Are you using the phone just to transport the files (write and read from the computer) and the additional files are showing up in...