NTFS should work fine although FAT32 is more widely accepted on varying system firmware. If the USB is formatted with the GPT partition scheme the firmware has to be set to UEFI and if it's formatted with MBR firmware needs to be in legacy or also referred to as CSM. All computers since the early 2000s are 64 bit capable so there really is no reason for a 32bit bootloader, but it should work regardless.