Hi again.
I assume it would but if you have more then 3 Gigabytes of ram you don't want to do that.
What is it that your are trying to do that requires virtual floppy drive?
If you have data on a lot of old floppy disks can you find someone with a older computer who could copy them to CD.
If you really need to run it, a better option might be to leave your Windows 7 64 bit alone, create a new partition and install 32 bit Windows XP or something compatible with VFD on it and run it from there.
Do the USB floppy drives available not work in Windows 7 64 bit, I don't see an answer to that.
I found this maybe this could help...
How to create a virtual floppy drive on Windows 7 64-bit - Super User
I found this floppy that says...